Double hairpin-assembled probe-mediating catalytic hairpin assembly and primer exchange reaction for sensitive and label-free miRNA analysis in gastric carcinoma

Abstract The aberrant expression of microRNA (miRNA) is closely associated with various pathological processes, such as the development gastric cancer. High-efficiency quantification miRNAs significant for diagnosis, prognosis, and treatment cancers. However, sensitive reliable detection miRNA remains a huge challenge. We depict here novel fluorescent approach label-free by exploiting designed scaffold to integrate catalytic hairpin assembly primer exchange reaction (PER). In this method, that constructed based on hybridization between two structure probes (H1 probe H2 probe), capable specifically recognizing target activating signal amplification, PER process transcribes numerous G-rich sequences induce ThT-based generation. Based efficient amplification strategy generation mode, method exhibits wide range 7 orders magnitude high repeatability (coefficient variation, 2.76%), implying proposed will be robust tool in early diagnosis disease. Graphical abstract